HORDB

General Information

ID: hor005868
Uniprot ID: Q6LEM5
Protein name: Bradykinin-potentiating peptide 6a
Gene name: NA
Organism: Bothrops jararaca (Jararaca) (Bothrops jajaraca)
Family: Bradykinin-potentiating peptide family; PHpG family; Natriuretic peptide family
Source: Animal
Expression: BPP-10a, BPP-10c+QQWA, BPP-13a, BPP-13+QWA and BPP-13A+QQWA seem to be found in both adult and newborn B.jararaca venoms. |Expressed in venom gland.
Disease: NA
Comments: NA
Taxonomy: Bothrops (genus), Crotalinae (subfamily), Viperidae (family), Colubroidea (superfamily), Serpentes (infraorder), Toxicofera , Episquamata , Unidentata , Bifurcata , Squamata (order), Lepidosauria (class), Sauria , Sauropsida , Amniota , Tetrapoda , Dipnotetrapodomorpha , Sarcopterygii (superclass), Euteleostomi , Teleostomi , Gnathostomata , Vertebrata , Craniata (subphylum), Chordata (phylum), Deuterostomia , Bilateria , Eumetazoa , Metazoa (kingdom), Opisthokonta , Eukaryota (superkingdom),cellular organisms
GO MF: GO:0004857 enzyme inhibitor activity; GO:0005179 hormone activity; GO:0008191 metalloendopeptidase inhibitor activity; GO:0030414 peptidase inhibitor activity; GO:0060422 peptidyl-dipeptidase inhibitor activity; GO:0090729 toxin activity
GO BP: GO:0006469 negative regulation of protein kinase activity; GO:0007165 signal transduction; GO:0008217 regulation of blood pressure; GO:0035821 modulation of process of another organism; GO:0042311 vasodilation; GO:0097746 blood vessel diameter maintenance
GO CC: GO:0005576 extracellular region; GO:0005737 cytoplasm; GO:0005829 cytosol

Sequence Information

Sequence: QSWPGP
Length: 6(31-36)
Propeptide: MVLSRLAASGLLLLALLALSVDGKPVQQWAQSWPGPNIPPLKVQQWAQGGWPRPGPEIPPLTVQQWAQNWPHPQIPPLTVQQWAQGRAPGPPIPPLTVQQWAQGRAPHPPIPPAPLQKWAPLQKWAPLLQPHESPASGTTALREELSLGPEAASGVPSAGAEVGRSGSKAPAAPHRLSKSKGAAATRPMRDLRPDGKQARQNWGRMAHHDHHAAAGGGGGGGGGARRLKGLAKKGAAKGCFGLKLDRIGTMSGLG
Signal peptide: MVLSRLAASGLLLLALLALSVDG
Modification: T1 Pyrrolidone carboxylic acid
Glycosylation: NA
Mutagenesis: 4-4P->A: Low decrease in ability to enhance AsS activity.; 6-6P->A: Low decrease in ability to enhance AsS activity.

Activity

Function: [Bradykinin-potentiating peptide 5a]: Modestly inhibits ACE (with highest affinity for the N-site) and reveals strong bradykinin-potentiating activity. Induces nitric oxide (NO) production depended on muscarinic acetylcholine receptor M1 subtype (CHRM1) a
Mechanism: Negative results: does not bind to type-1 angiotensin-2 receptor (AGTR1).
Cross BBB: NA
Target: NA
Target Unid: NA
IC50: NA
EC50: NA
ED50: NA
kd: NA
Half life: NA

Structure

Disulfide bond: NA
Structure ID: AF-Q6LEM5-F1(AlphaFold_DB_ID)

Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)

hor005868_AF2.pdb

hor005868_ESM.pdb

Physical Information

Mass:	76008	Formula:	C31H42N8O9
Absent amino acids:	ACDEFHIKLMNRTVY	Common amino acids:	P
pI:	6.11	Basic residues:	0
Polar residues:	2	Hydrophobic residues:	1
Hydrophobicity:	-146.67	Boman Index:	-567
Half-Life:	0.8 hour	Half-Life Yeast:	10 min
Half-Life E.Coli:	>10 hour	Aliphatic Index	0
Instability Index:	8156.67	Extinction Coefficient cystines:	5500
Absorbance 280nm:	1100

Literature

PubMed ID: 9037028
Title: Cloning and sequence analysis of a Bothrops jararaca cDNA encoding a precursor of seven bradykinin-potentiating peptides and a C-type natriuretic peptide.
PubMed ID: 4334402
Title: Angiotensin-converting enzyme inhibitors from the venom of Bothrops jararaca. Isolation, elucidatio
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